Team:Paris Bettencourt/Notebook/TB-ception/Tuesday 10th September.html

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TB-ception

Tuesday 10th July


PCR for the pSB3C5 and pSB1C3 backbone with the oligos for building a new backbone. The oligos are --> pDCA reverse with pDCA1/2/3 forward; pDCo4 reverse and forward. Gel slice and gel purification. Passaged the macrophages.