Team:Groningen/Labwork/15 September 2013

Claudio

Plates containing pSB1C3-S1-S5-S5-S13 and pSB1C3-S2-S5-S5-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
Plates containing pSB1C3-S16-S3-S5-S13 and pSB1C3-S16-S9-S11-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Colony ?? from ?? and colony ?? from ?? show to be positive candidates and were inoculated in LB + Cm.

Colony PCR (NEB Master Mix) was performed on 4 colonies from plate pSB1C3-S2-S5-S14 using VF2 and VR primers (annealing temperature 65°C).
Colony PCR (NEB Master Mix) was performed on 4 colonies from plate BBa_K1085014-S1-S5-S14 using F_spank_NheI and HM14 primers (annealing temperature 68°C).
The samples were checked on agarose gel 0.8%.

Colony ?? from plate ?? showed a band at the expected height (~2400 bps). Therefore was inoculated in LB + Cm.
Colony ?? from plate ?? showed a band at the expected height (~2400 bps). Therefore was inoculated in LB + Cm.