Team:Peking/projecttest
From 2013.igem.org
Biosensors
A FAST, EASY AND ACCURATE METHOD TO DETECT TOXIC AROMATIC COMPOUNDS
NahR
Overview
The nahR gene originated from the 83 kb naphthalene degradation plasmid NAH7 of Pseudomonas putida encodes a 34 kDa protein which binds to nah and sal promoters to activate transcription of the degradation genes in response to the inducer salicylate. This plasmid encodes enzymes for the metabolism of naphthalene or salicylate as the sole carbon source (Fig. 1a) [1]. The 14 genes encoding the enzymes for this metabolism are organized in two operons: nah (nahA-F), encoding six enzymes required for metabolism for naphthalene to salicylate and pyruvate, and sal (nahG-M), encoding eight enzymes which metabolize salicylate to intermediates of TCA cycle (Fig. 1b) [2].