Team:DTU-Denmark/Notebook/11 June 2013

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11 June 2013

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Contents

208 lab

Main purposes today

helloworld-project

  • diluting primers with 1.5 ml milli Q water each
  • diluting G-block with 20ul milli Q water

Who was in the lab

Procedure

PCR

  • 7 rxns in duplicates
  • mastermix for 15 rxns
per rxnmaster
5x HF buffer10ul150ul
MgCl_20.5ul7.5ul
dNTPs5ul75ul
Phusion polymerase0.5ul7.5ul
Milli Q water25ul375ul
master mix total 615 ul

Every PCR tube contains

mastermix41ul
FW primer3ul
RV primer3ul
DNA template1ul
PCR tube total 50ul

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