lab 208

Main purpose

Who was in the lab

Procedure

Colony PCR to verify Nir in pZA21::ara-tight

Colony PCR with sequencing primers Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.

Re-run of yesterday's PCR with the following new annealing temperatures: pair 1: 67C, pair 2: 68C

Results

Gels

• 1 kb
• colony 1, HAO
• colony 2, HAO
• colony 3, HAO
• colony 4, HAO
• colony 5, HAO
• colony 6, HAO
• Nir colony 7, primer pair 1
• Nir colony 7, primer pair 2
• Nir colony 8, primer pair 1
• Nir colony 8, primer pair 2
• 1 kb

Made new PCR with higher annealing temperature to obliterate unspecific binding.

• 1 kb
• Nir colony 1, primer pair 1
• Nir colony 1, primer pair 1
• Nir colony 1, primer pair 2
• Nir colony 1, primer pair 2

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