From 2013.igem.org
Aug 23
Electrophoresis
No name
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | pT181 antisense | EcoRI | SpeI
|
| 2 | pT181 antisense | EcoRI | --
|
| 3 | pT181 antisense | -- | SpeI
|
| 4 | pT181 antisense | -- | --
|
| 5 | pT181 antisense | XbaI | PstI
|
| 6 | pT181 antisense | XbaI | --
|
| 7 | pT181 antisense | -- | PstI
|
| 8 | pT181 antisense | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | Fusion1 attenuator | EcoRI | SpeI
|
| 11 | Fusion1 attenuator | EcoRI | --
|
| 12 | Fusion1 attenuator | -- | SpeI
|
| 13 | Fusion1 attenuator | -- | --
|
| 14 | Fusion1 attenuator | XbaI | PstI
|
| 15 | Fusion1 attenuator | XbaI | --
|
| 16 | Fusion1 attenuator | -- | PstI
|
| 17 | Fusion1 attenuator | -- | --
|
File:Igku xxxxxx.xxx
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | Fusion3m2 attenuator | EcoRI | SpeI
|
| 2 | Fusion3m2 attenuator | EcoRI | --
|
| 3 | Fusion3m2 attenuator | -- | SpeI
|
| 4 | Fusion3m2 attenuator | -- | --
|
| 5 | Fusion3m2 attenuator | XbaI | PstI
|
| 6 | Fusion3m2 attenuator | XbaI | --
|
| 7 | Fusion3m2 attenuator | -- | PstI
|
| 8 | Fusion3m2 attenuator | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | tetR aptamer 12_1M | EcoRI | SpeI
|
| 11 | tetR aptamer 12_1M | EcoRI | --
|
| 12 | tetR aptamer 12_1M | -- | SpeI
|
| 13 | tetR aptamer 12_1M | -- | --
|
| 14 | tetR aptamer 12_P | EcoRI | SpeI
|
| 15 | tetR aptamer 12_P | EcoRI | --
|
| 16 | tetR aptamer 12_P | -- | SpeI
|
| 17 | tetR aptamer 12_P | -- | --
|
File:Igku xxxxxx.xxx
Kojima and Honda
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | Plac | SpeI | PstI
|
| 2 | Plac | SpeI | --
|
| 3 | Plac | -- | PstI
|
| 4 | Plac | -- | --
|
| 5 | Spinach | EcoRI | SpeI
|
| 6 | Spinach | EcoRI | --
|
| 7 | Spinach | -- | SpeI
|
| 8 | Spinach | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | J23100 | SpeI | PstI
|
| 11 | J23100 | SpeI | --
|
| 12 | J23100 | -- | PstI
|
| 13 | J23100 | -- | --
|
| 14 | tetR aptamer 12_1R | EcoRI | SpeI
|
| 15 | tetR aptamer 12_1R | EcoRI | --
|
| 16 | tetR aptamer 12_1R | -- | SpeI
|
| 17 | tetR aptamer 12_1R | -- | --
|
File:Igku xxxxxx.xxx
Restriction Enzyme Digestion
Electrophoresis
"
