From 2013.igem.org
Aug 23
Electrophoresis
No name
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | pT181 antisense | EcoRI | SpeI
|
| 2 | pT181 antisense | EcoRI | --
|
| 3 | pT181 antisense | -- | SpeI
|
| 4 | pT181 antisense | -- | --
|
| 5 | pT181 antisense | XbaI | PstI
|
| 6 | pT181 antisense | XbaI | --
|
| 7 | pT181 antisense | -- | PstI
|
| 8 | pT181 antisense | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | Fusion1 attenuator | EcoRI | SpeI
|
| 11 | Fusion1 attenuator | EcoRI | --
|
| 12 | Fusion1 attenuator | -- | SpeI
|
| 13 | Fusion1 attenuator | -- | --
|
| 14 | Fusion1 attenuator | XbaI | PstI
|
| 15 | Fusion1 attenuator | XbaI | --
|
| 16 | Fusion1 attenuator | -- | PstI
|
| 17 | Fusion1 attenuator | -- | --
|
File:Igku xxxxxx.xxx
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | Fusion3m2 attenuator | EcoRI | SpeI
|
| 2 | Fusion3m2 attenuator | EcoRI | --
|
| 3 | Fusion3m2 attenuator | -- | SpeI
|
| 4 | Fusion3m2 attenuator | -- | --
|
| 5 | Fusion3m2 attenuator | XbaI | PstI
|
| 6 | Fusion3m2 attenuator | XbaI | --
|
| 7 | Fusion3m2 attenuator | -- | PstI
|
| 8 | Fusion3m2 attenuator | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | tetR aptamer 12_1M | EcoRI | SpeI
|
| 11 | tetR aptamer 12_1M | EcoRI | --
|
| 12 | tetR aptamer 12_1M | -- | SpeI
|
| 13 | tetR aptamer 12_1M | -- | --
|
| 14 | tetR aptamer 12_P | EcoRI | SpeI
|
| 15 | tetR aptamer 12_P | EcoRI | --
|
| 16 | tetR aptamer 12_P | -- | SpeI
|
| 17 | tetR aptamer 12_P | -- | --
|
File:Igku xxxxxx.xxx
Kojima and Honda
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | Plac | SpeI | PstI
|
| 2 | Plac | SpeI | --
|
| 3 | Plac | -- | PstI
|
| 4 | Plac | -- | --
|
| 5 | Spinach | EcoRI | SpeI
|
| 6 | Spinach | EcoRI | --
|
| 7 | Spinach | -- | SpeI
|
| 8 | Spinach | -- | --
|
| 9 | 100bp ladder | -- | --
|
| 10 | J23100 | SpeI | PstI
|
| 11 | J23100 | SpeI | --
|
| 12 | J23100 | -- | PstI
|
| 13 | J23100 | -- | --
|
| 14 | tetR aptamer 12_1R | EcoRI | SpeI
|
| 15 | tetR aptamer 12_1R | EcoRI | --
|
| 16 | tetR aptamer 12_1R | -- | SpeI
|
| 17 | tetR aptamer 12_1R | -- | --
|
File:Igku xxxxxx.xxx
Restriction Enzyme Digestion
Nakamoto and Kojima
| Fusion6 antisense (1) | EcoRI | SpeI | buffer B | BSA | MilliQ | total
|
| 3 | 0.5 | 0.5 | 3 | 0.3 | 22.7 | 30
|
| 0.6 | 0.1 | 0 | 1 | 0.1 | 8.2 | 10
|
| 0.6 | 0 | 0.1 | 1 | 0.1 | 8.2 | 10
|
| 0.6 | 0 | 0 | 1 | 0.1 | 8.3 | 10
|
| Fusion6 antisense (1) | XbaI | PstI | buffer D | BSA | MilliQ | total
|
| 3 | 0.5 | 0.5 | 3 | 0.3 | 22.7 | 30
|
| 0.6 | 0.1 | 0 | 1 | 0.1 | 8.2 | 10
|
| 0.6 | 0 | 0.1 | 1 | 0.1 | 8.2 | 10
|
| 0.6 | 0 | 0 | 1 | 0.1 | 8.3 | 10
|
| Fusion1 antisense (1) | EcoRI | SpeI | buffer B | BSA | MilliQ | total
|
| 3.3 | 0.5 | 0.5 | 3 | 0.3 | 22.4 | 30
|
| 0.7 | 0.1 | 0 | 1 | 0.1 | 8.1 | 10
|
| 0.7 | 0 | 0.1 | 1 | 0.1 | 8.1 | 10
|
| 0.7 | 0 | 0 | 1 | 0.1 | 8.2 | 10
|
| pT181 antisense (1) | EcoRI | SpeI | buffer B | BSA | MilliQ | total
|
| 5.6 | 0.5 | 0.5 | 3 | 0.3 | 20.1 | 30
|
| 1.1 | 0.1 | 0 | 1 | 0.1 | 7.7 | 10
|
| 1.1 | 0 | 0.1 | 1 | 0.1 | 7.7 | 10
|
| 1.1 | 0 | 0 | 1 | 0.1 | 7.8 | 10
|
| pT181 antisense (1) | XbaI | PstI | buffer D | BSA | MilliQ | total
|
| 5.6 | 0.5 | 0.5 | 3 | 0.3 | 20.1 | 30
|
| 1.1 | 0.1 | 0 | 1 | 0.1 | 7.7 | 10
|
| 1.1 | 0 | 0.1 | 1 | 0.1 | 7.7 | 10
|
| 1.1 | 0 | 0 | 1 | 0.1 | 7.8 | 10
|
- incubate at 37°C from 17:29.
Electrophoresis
No name
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | 100bp | -- | --
|
| 2 | pT181 attenuator | EcoRI | SpeI
|
| 3 | pT181 attenuator | XbaI | PstI
|
| 4 | Fusion3m2 attenuator | EcoRI | SpeI
|
| 5 | Fusion1 attenuator | XbaI | PstI
|
| 6 | Fusion1 attenuator | EcoRI | SpeI
|
| 7 | Fusion3m2 attenuator | XbaI | PstI
|
| 8 | pT181 antisense | EcoRI | SpeI
|
| 9 | pT181 antisense | XbaI | PstI
|
| 10 | Fusion1 antisense | EcoRI | SpeI
|
| 11 | Fusion1 antisense | XbaI | PstI
|
| 12 | Fusion6 antisense | EcoRI | SpeI
|
| 13 | Fusion6 antisense | XbaI | PstI
|
| 14 | tetR aptamer 12_1M | EcoRI | SpeI
|
| 15 | tetR aptamer 12_P | EcoRI | SpeI
|
| 16 | tetR aptamer 12_1R | EcoRI | SpeI
|
| 17 | 100bp | -- | --
|
File:Igku xxxxxx.xxx
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | 100bp | -- | --
|
| 2 | Spinach | EcoRI | SpeI
|
| 3 | J23100 | SpeI | PstI
|
| 4 | Plac | SpeI | PstI
|
| 5 | 100bp | -- | --
|
File:Igku xxxxxx.xxx
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | 100bp | -- | --
|
| 2 | Fusion6 antisense | EcoRI | SpeI
|
| 3 | Fusion6 antisense | EcoRI | --
|
| 4 | Fusion6 antisense | -- | SpeI
|
| 5 | Fusion6 antisense | -- | --
|
| 6 | pT181 antisense | XbaI | PstI
|
| 8 | 100bp | -- | --
|
| 9 | 100bp | -- | --
|
| 10 | Fusion6 antisense | XbaI | PstI
|
| 11 | Fusion6 antisense | XbaI | --
|
| 12 | Fusion6 antisense | -- | PstI
|
| 13 | Fusion6 antisense | -- | --
|
| 14 | pT181 antisense | XbaI | --
|
| 16 | 100bp | -- | --
|
File:Igku xxxxxx.xxx
| Lane | Sample | Enzyme1 | Enzyme2
|
| 1 | 100bp | -- | --
|
| 2 | Fusion1 antisense | EcoRI | SpeI
|
| 3 | Fusion1 antisense | EcoRI | --
|
| 4 | Fusion1 antisense | -- | SpeI
|
| 5 | Fusion1 antisense | -- | --
|
| 6 | pT181 antisense | -- | PstI
|
| 8 | 100bp | -- | --
|
| 9 | 100bp | -- | --
|
| 10 | pT181 antisense | EcoRI | SpeI
|
| 11 | pT181 antisense | EcoRI | --
|
| 12 | pT181 antisense | -- | SpeI
|
| 13 | pT181 antisense | -- | --
|
| 14 | pT181 antisense | -- | --
|
| 16 | 100bp | -- | --
|
File:Igku xxxxxx.xxx
Nakamoto, Ashida, Kojima, Okazaki & Matsumoto
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | pT181 attenuator | XbaI & PstI
|
| 3
|
| 5 | Fusion3m2 attenuator | EcoRI & SpeI
|
| 6
|
| 8 | Fusion1 attenuator | XbaI & PstI
|
| 9
|
| 11 | Fusion1 attenuator | EcoRI & SpeI
|
| 12
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| 1 | | | |
|
| （ΦωΦ^） | | | |
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | Fusion3m2 attenuator | XbaI & PstI
|
| 3
|
| 5 | pT181 antisense | EcoRI & SpeI
|
| 6
|
| 8 | pT181 antisense | XbaI & PstI
|
| 9
|
| 11 | Fusion1 antisense | XbaI & PstI
|
| 12
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| 1 | | | |
|
| （ΦωΦ^） | | | |
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | Fusion6 attenuator | XbaI & PstI
|
| 3
|
| 5 | tetR aptamer 12_1M | EcoRI & SpeI
|
| 6
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | tetR aptamer 12_P | EcoRI & SpeI
|
| 3
|
| 5 | tetR aptamer 12_1R | EcoRI & SpeI
|
| 6
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| 1 | | | |
|
| （ΦωΦ^） | | | |
|
Ashida
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | Fusion6 antisense | EcoRI & SpeI
|
| 3
|
| 5 | Fusion6 attenuator | XbaI & PstI
|
| 6
|
| 8 | Fusion1 antisense | EcoRI & SpeI
|
| 9
|
| 11 | pT181 antisense | EcoRI & SpeI
|
| 12
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| 1 | | | |
|
| （ΦωΦ^） | | | |
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 3 | pT181 antisense | XbaI & PstI
|
| 4
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| 1 | | | |
|
| （ΦωΦ^） | | | |
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 3 | Plac | SpeI & PstI
|
| 4
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 3 | J23100 | SpeI & PstI
|
| 5 | Spinach | EcoRI & SpeI
|
| 6
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| 1 | | | |
|
| （ΦωΦ^） | | | |
|
"
