Template:Kyoto/Notebook/Aug 24

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Contents

Aug 24

Gel Extraction

Ashida

Nameconcentration[µg/mL]260/280260/230
8/22 aptamer 12_P (EcoRI & SpeI)8821.3--
8/22 aptamer 12_1R (EcoRI & SpeI)6323.1--
8/22 aptamer 12_1M (EcoRI & SpeI)4022.0--
8/23 Fusion6 antisense (EcoRI & SpeI)6.01.600.17
8/23 Fusion6 antisense (XbaI & PstI)6.01.710.25
8/23 pT181 antisense (EcoRI & SpeI)6.02.220.15
8/23 pT181 antisense (XbaI & PstI)6.02.100.04
8/22 Plac (SpeI & PstI)6.01.470.09
8/22 Fusion1 antisense (EcoRI & SpeI)102.160.01
8/22 J23100 (SpeI & PstI)6.01.520.35
Nameconcentration[µg/mL]260/280260/230
8/22 Spinach (EcoRI & SpeI)8.01.150.77
8/22 Fusion6 antisense (XbaI & PstI)441.070.96
8/22 Fusion1 antisense (XbaI & PstI)2--1.00
8/22 pT181 antisense (XbaI & PstI)3.2.010.40
8/22 pT181 antisense (EcoRI & SpeI)81.130.82
8/22 Fusion3m2 attenuator (XbaI & PstI)52.2771.50
8/22 Fusion3m2 attenuator (EcoRI & SpeI)341.161.02
8/22 Fusion1 attenuator (XbaI & PstI)141.400.62
8/22 Fusion1 attenuator (EcoRI & SpeI)161.270.61
8/22 pT181 attenuator (XbaI & PstI)5--0.88
  • These samples were performed ethanol ethanl precipitation.

Restriction Enzyme Digestion

No name

tetR aptamer 12_P(2)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2.7µL0.5µL0.5µL3µL0.3µL23µL30µL
0.3µL0µL0µL1µL0.1µL8.6µL10µL
tetR aptamer 12_1R(2)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2.6µL0.5µL0.5µL3µL0.3µL23.1µL30µL
0.3µL0µL0µL1µL0.1µL8.6µL10µL
tetR aptamer 12_M(1)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2.7µL0.5µL0.5µL3µL0.3µL23µL30µL
0.3µL0µL0µL1µL0.1µL8.6µL10µL

Master Plate

No name

NumberUse LB plate(+Kan)
1entA- #1
2entA- #1
3entA- #1
4entA- #2
5entA- #2
6entA- #2

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
11oobp ladder--
2tetR aptamer 12_PEcoRISpeI
3tetR aptamer 12_P--
4tetR aptamer 12_1REcoRISpeI
5tetR aptamer 12_1R--
6tetR aptamer 12_1MEcoRISpeI
7tetR aptamer 12_1M--
81oobp ladder--
LaneSampleEnzyme1Enzyme2
11oobp ladder--
2tetR aptamer 12_PEcoRISpeI
3tetR aptamer 12_1REcoRISpeI
4tetR aptamer 12_1MEcoRISpeI
51oobp ladder--

Gel Extraction

Matsumoto and Ashida

LaneDNAEnzyme
1100bp ladder-
28/24 tetR aptamer 12_PEcoRI&SpeI
48/24 tetR aptamer 12_1REcoRI&SpeI
68/24 tetR aptamer 12_MEcoRI&SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
tetR aptamer 12_P(EcoRI&SpeI)2.30.380.13
tetR aptamer 12_1R(EcoRI&SpeI)12.00.631.19
tetR aptamer 12_M(EcoRI&SpeI)2.06.430.03
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