Template:Kyoto/Notebook/Aug 27

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Aug 27

Restriction Enzyme Digestion

No name

8/21 pT181 attenuator-1(330µg/mL)

EcoRI

SpeI

10xBufferB

100xBSA

MilliQ

total

3.0µL

0.5µL

0.5µL

3.0µL

0.3µL

22.7µL

30µL

0.6µL

0µL

0µL

1µL

0.1µL

8.3µL

10µL

8/17 DT-1(188µg/mL)	EcoRI	XbaI	10xBufferB	100xBSA	MilliQ	total
3.0µL	0.5µL	0.5µL	3.0µL	0.3µL	22.7µL	30µL
1.0µl	0µL	0µL	1µL	0.1µL	7.9µL	10µL

8/20 Pcon-RBS-luxR-DT-2(344µg/mL)	EcoRI	SpeI	10xBufferB	100xBSA	MilliQ	total
2.9µL	0.5µL	0.5µL	3.0µL	0.3µL	22.8µL	30µL
0.6µl	0µL	0µL	1µL	0.1µL	8.3µL	10µL

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