Template:Kyoto/Notebook/Aug 27
From 2013.igem.org
Aug 27
Restriction Enzyme Digestion
8/21 pT181 attenuator-1(330µg/mL) | EcoRI | SpeI | 10xBufferB | 100xBSA | MilliQ | total |
---|---|---|---|---|---|---|
3.0µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.7µL | 30µL |
0.6µL | 0µL | 0µL | 1µL | 0.1µL | 8.3µL | 10µL |
8/17 DT-1(188µg/mL) | EcoRI | XbaI | 10xBufferB | 100xBSA | MilliQ | total |
---|---|---|---|---|---|---|
3.0µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.7µL | 30µL |
1.0µl | 0µL | 0µL | 1µL | 0.1µL | 7.9µL | 10µL |
8/20 Pcon-RBS-luxR-DT-2(344µg/mL) | EcoRI | SpeI | 10xBufferB | 100xBSA | MilliQ | total |
---|---|---|---|---|---|---|
2.9µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.8µL | 30µL |
0.6µl | 0µL | 0µL | 1µL | 0.1µL | 8.3µL | 10µL |