Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 19th July.html

Trojan Horse

ASDF

19^th July

Place your twit here

Conjugation results :

Controls => no colonies

Mix = tapis

Ie worked really well.
To be sure to start only with one clone => restreak some colonies on tet/spec plates

Transformation by electroporation : goal = get a phage factory.

We do it three different ways in order to see which one works (best).

Transform strain containing helper plasmid with phagemid

Transform strain containing helper plasmid with phagemid

Co Transform strain with both plasmids at the same time

From strain sT004, sT005, sT006

Transform sT004 with pT006

Transform sT005 with pT005

Co Transform sT006 with pT005 and pT006

Tubes :

1. sM13KO7 (control) : 5,60
2. sM13KO7 + pLitmus : 5,70
3. sM13KO7 + pLitmus : 5,70
4. sLITMUS (control) : 5,60
5. sLITMUS + pM13K07 : 5,60
6. sLITMUS + pM13K07 : 5,60
7. MG16165 : control = 5.70
8. MG16165 + p (M13K07) and p(LITMUS) = 5.60
9. MG16165 + p (M13K07) and p(LITMUS)= 5.60

Recovery 2h

Plate everything on Kan/ Amp