Team:Paris Bettencourt/Notebook/Drug Screening/Thursday 19th September.html

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Drug Screening

Thursday 19th September

PCR Verification of vectors

Vectors (pET FprA FdxA, pSB1c3 SirA, and pSB1c3 FprA) were used for colony PCR using DreamTaq. Primers used were:

pET FprA FdxA: MCSI F/R, MCSII F/R, FprA F/R, FdxA F/R

pSB1c3 SirA: SB-prep-3P-1/SB-prep-2Ea, SirA F/R

pSB1c3 FprA: SB-prep-3P-1/SB-prep-2Ea, FprA F/R

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