From 2013.igem.org
Aug 28
Electrophoresis
Miniprep
No name
DNA | concentration[µg/mL] | 260/280 | 260/230
|
8/27 Plux | 173.8 | 1.95 | 1.45
|
Ligation
No name
state | Vector | Inserter
|
experiment | 8/71 DT-1 | 3.0 | 8.0
|
- Samples were evaporeted used evaporator into about 3 µL.
sample | MilliQ | Ligation High | total
|
3 | 4 | 3.5 | 10.5
|
- incubate one hour at 16 °C
No name
Lane | DNA
|
1 | 100bpladder | --
|
3 | SasA(8/27 Genome PCR production)
|
5 | RpaA(8/27 Genome PCR production)
|
7 | RpaB(8/27 Genome PCR production)
|
9 | PkaiBC(8/27 Genome PCR production)
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Colony PCR
No name
Sample | base pair
|
8/21 RBS-lysis2-(3) | 772
|
8/21 RBS-lysis2-(4) | 772
|
8/21 RBS-lysis2-(5) | 772
|
8/21 RBS-lysis2-(6) | 772
|
PreDenature | Denature | Annealing | Extension | cycle
|
94°C | 94°C | 55°C | 68°C | --
|
5min | 30s | 30s | 48s | 30cycles
|
File:Igku Aug19electrophoresis1.png
Restriction Enzyme Digestion
No name
| 8/22 RBS-lysis1-(1) | EcoRI | SpeI | BufferB | BSA | MilliQ | total
|
2 cuts | 9µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 16.7µL | 30µL
|
NC | 1µL | 0µL | 0µL | 1µL | 0.1µL | 7.9µL | 10µL
|
| 8/17 RBS-GFP-DT-(2) | XbaI | PstI | BufferD | BSA | MilliQ | total
|
2 cuts | 4µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 21.7µL | 30µL
|
NC | 0.5µL | 0µL | 0µL | 1µL | 0.1µL | 8.4µL | 10µL
|
| 8/20 Pcon-RBS-GFP-DT-(1) | EcoRI | SpeI | BufferB | BSA | MilliQ | total
|
2 cuts | 3µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 22.7µL | 30µL
|
NC | 0.3µL | 0µL | 0µL | 1µL | 0.1µL | 8.6µL | 10µL
|
| 8/20 Pcon-RBS-luxR-DT-(1) | EcoRI | XbaI | BufferD | BSA | MilliQ | total
|
2 cuts | 2µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 23.7µL | 30µL
|
NC | 0.3µL | 0µL | 0µL | 1µL | 0.1µL | 8.6µL | 10µL
|
No name
| 8/28 Plux | SpeI | PstI | BufferD | BSA | MilliQ | total
|
2 cuts | 6µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 19.7µL | 30µL
|
NC | 1µL | 0µL | 0µL | 1µL | 0.1µL | 7.9µL | 10µL
|
| 8/20 RBS-tetR-DT-(1) | XbaI | PstI | BufferD | BSA | MilliQ | total
|
2 cuts | 5µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 20.7µL | 30µL
|
NC | 0.5µL | 0µL | 0µL | 1µL | 0.1µL | 8.4µL | 10µL
|
Electrophoresis
Colony PCR
Liquid Culture
Ligation
===Liquid Culture===