From 2013.igem.org
Aug 29
Colony PCR
No name
| Sample | base pair
|
| 8/27 Pcon-pT181 antisense-(1) | 542
|
| 8/26 pT181 attenuator-DT-(1) | 738
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 30s | 30cycles
|
File:Igku Aug19electrophoresis1.png
Electrophoresis
No name
| Lane | Sample
|
| 1 | 100bp ladder
|
| 2 | 8/21 RBS-lysis2-9(Colony PCR prodution)
|
| 3 | 8/21 RBS-lysis2-8(Colony PCR prodution)
|
| 4 | 8/21 RBS-lysis2-7(Colony PCR prodution)
|
| 5 | 8/21 RBS-lysis2-10(Colony PCR prodution)
|
| 6 | 8/21 RBS-lysis2-11(Colony PCR prodution)
|
| 7 | 8/21 RBS-lysis2-12(Colony PCR prodution)
|
| 8 | 8/21 RBS-lysis2-13(Colony PCR prodution)
|
| 9 | 8/21 RBS-lysis2-14(Colony PCR prodution)
|
File:Igku xxxxxx.xxx
miniprep
No Name
| DNA | concentration [µg/mL] | 260/280 | 260/230
|
| Plac | 153.7 | 2.03 | 2.10
|
| Pλ-luxⅠ-(1) | 76.5 | 2.07 | 2.06
|
| Pλ-luxⅠ-(2) | 86.4 | 2.08 | 2.10
|
| DT | 166.2 | 1.65 | 2.12
|
| J23100 | 190.1 | 1.68 | 2.34
|
Liquid Culture
No name
| Sample | medium
|
| 8/21 RBS-Lysis2-9 | Plusgrow medium(+Amp)
|
| RBS-Lysis2-12 | Plusgrow medium(+Amp)
|
| RBS-Lysis2-14 | Plusgrow medium(+Amp)
|
Restriction Enzyme Digestion
Transformation
M9 Medium Plate
"
