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Team:Kyoto/Safety
From 2013.igem.org
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Use this page to answer the questions on the safety page.
1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:
| Species | Strain no/name | Risk Group | Risk group source link | Disease risk to humans? If so, which disease? | |
|---|---|---|---|---|---|
| 1 | E. coli (K 12) | JW0588 | 1 | www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 2 | E. coli (K 12) | JM109 | 1 | www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 3 | E. coli (K 12) | XL10-gold | 1 | www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 4 | E. coli (K 12) | BL(DE3)pLysS | 1 | www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
2. Highest Risk Group Listed: 1
3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)
| Part number. | Where did you get the physical DNA for this part (which lab, synthesis company, etc) | What species does this part originally come from? | What is the Risk Group of the species? | What is the function of this part, in its parent species? | |
|---|---|---|---|---|---|
| 1 | BBa_K1126000 | Synthesized, IDT | in vitro selection | 1 | induces TetR-controlled gene expression |
| 2 | BBa_K1126001 | Synthesized, IDT | in vitro selection | 1 | induces TetR-controlled gene expression |
| 3 | BBa_K1126002 | Synthesized, IDT | in vitro selection | 1 | induces TetR-controlled gene expression |
| 4 | BBa_K1126003 | Synthesized, IDT | Staphylococcus aureus | 1 | regulates transcription with antisense |
| 5 | BBa_K1126004 | Synthesized, IDT | Staphylococcus aureus | 1 | regulates transcription with antisense |
| 6 | BBa_K1126005 | Synthesized, IDT | Staphylococcus aureus | 1 | regulates transcription with antisense |
| 7 | BBa_K1126006 | Synthesized, IDT | Staphylococcus aureus | 1 | regulates transcription with attenuator |
| 8 | BBa_K1126007 | Synthesized, IDT | Staphylococcus aureus | 1 | regulates transcription with attenuator |
| 9 | BBa_K1126008 | Synthesized, IDT | Staphylococcus aureus | 1 | regulates transcription with attenuator |
