Template:Kyoto/Notebook/Aug 30

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Contents

Aug 30

Gel Extraction

No Name

Namequantityconcentration[µg/mL]260/280260/230
PT181 atte(2) Ets305.3 2.2 1.66 0.36
PT181 atte(2) Xtp217.7 3.9 1.73 0.04
PT181 anti(2) Ets347.7 3.1 1.88 0.31
PT181 anti(2) Xtp376.5 5.9 0.49 2.36
PSBIC3 Ets436.6 30.7 1.03 1.28
PSBIC3 Xtp457.4 7.9 1.65 0.42
Spinach Ets490.5 3.0 1.87 0.26
tetR aptamer 12-1R Ets451.7 -12.8 5.56 -0.04

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
RBS-lysis2 9264.6　1.97　2.21
RBS-lysis2 12220.0　1.89　2.46
RBS-lysis2 14195.3　1.88　1.53

Colony PCR

No name

Samplebase pair
8/29 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(8/20 LB Amp) (1)~(4)2143
8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)1227
8/29 Pbad-larac-RBS-RFP{BBaI13516} (8/21 LB-CP) (1)~(2)2256
8/29 RBS-lysis3-DT-(1)1210
PreDenatureDenatureAnnealingExtensioncycle
　94°C　94°C　55°C　68°C--
5min　30s　30s　2min　30cycle

Ethanol Precipitation

No name

DNAconcentration[µg/mL]260/280260/230
pT181 atte-(2) Et3
pT181 atte-(2) Xtp
pT181 anti-(2) Et3
pT181 anti-(2) Xtp
pSB1C3 Et3
pSB1C3 Xtp
Spinach Et3
tetR aptamer 12_1R Et3
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