From 2013.igem.org

• Home
• Plan
• Protocols
• Notebook
  • E. coli
  • Methanococcus
• Results
• Parts
• Safety
• Outreach
  • Engineering Seminar
  • Clarke Middle School
• Human Practice
• The Team
  • Meet The Team
  • Official Team Profile
• Acknowledgements

Protocols

E. coli Lab

1. Plasmid extraction: ZYMO RESEARCH [http://www.zymoresearch.com/media/downloads/47/D4036i.pdf]

2. PCR: KOD/ Phusion Polymerase (EMD Chemicals Inc.,NJ) (Novagen Protocol)/ NEB Manufacturer’s kit

3. Restriction Digestion: New England Biolabs (Beverly, MA) Manufacturer’s kit

4. Ligation: Rapid DNA Ligase (Roche Applied Science, Indianapolis, IN). Manufacturer’s kit

5. Chemical competent cell and Transformation (Heat shock) (Protocol developed by hands on experience)

6. PCR product purification kit and gel extraction kit (ZYMO RESEARCH)

Detailed Protocols for Methanococcus Lab is listed in the notebook [1]