From 2013.igem.org
Aug 31
No name
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | -- | --
|
| 3 | RBS-lysis2-(9) | EcoRI & SpeI
|
| 4
|
| 5 | -- | --
|
| 6 | pSB1C3(1) | EcoRI & SpeI
|
| 7
|
| 8 | -- | --
|
| 9 | pSB1C3(1) | XbaI & PstI
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | tRNA-Spinach | EcoRI & SpeI
|
| 3
|
| 4 | -- | --
|
| 5 | pT181 attenuator-(2) | EcoRI & SpeI
|
| 6
|
| 7 | pT181 attenuator-(2) | XbaI & PstI
|
| 8
|
| 9 | -- | --
|
| 10 | pT181 antisense-(2) | EcoRI & SpeI
|
| 11
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | pT181 antisense | XbaRI & PstI
|
| 3
|
| 4 | -- | --
|
| 5 | tetR aptamer 12_1R-(1) | EcoRI & SpeI
|
| 6
|
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| RBS-lysis2(EcoRI&SpeI) | 4.0 | 2.69 | 0.24
|
| pSB1C3(EcoRI&SpeI) | 6.0 | 2.48 | 0.05
|
| pSB1C3(XbaI&PstI) | -- | -- | --
|
| tRNA-Spinach | -- | -- | --
|
| pT181 attenuator-(2)(EcoRI&SpeI) | -- | -- | --
|
| pT181 anttenuator-(2)(SbaI&PstI) | -- | -- | --
|
| pT181 antisense-(2)(EcoRI&SpeI) | -- | -- | --
|
| pT181 antisense-(2)(XbaI&PstI) | -- | -- | --
|
| tetR aptamer 12_1R(EcoRI&SpeI) | -- | -- | --
|
Ligation
Nakamoto and Hirano
| state | Vector | Inserter | Ligation High ver.2
|
| experiment | 8/30 pSB1C3 (EcoRI & SpeI) | 6.6 | 8/30 tRNA-Spinach (EcoRI & SpeI) | 12.5 | 3.5
|
| experiment | 8/30 pSB1C3 (EcoRI & SpeI) | 6.6 | 8/30 pT181 antisense (EcoRI & SpeI) | 12.5 | 3.5
|
| experiment | 8/30 pSB1C3 (XbaI & Pst) | 12.5 | 8/30 pT181 attenuator (XbaI & PstI) | 25 | 3.5
|
| experiment | 8/30 pSB1C3 (XbaI & Pst) | 12.5 | 8/30 pT181 antisense (XbaI & PstI) | 25 | 3.5
|
| experiment | 8/20 DT (EcoRI & XbaI) | 6.6 | 8/30 pT181 attenuator(2) (EcoRI & SpeI) | 12.5 | 3.5
|
| experiment | 8/20 DT (EcoRI & XbaI) | 6.6 | 8/30 pT181 antisense (EcoRI & SpeI) | 12.5 | 3.5
|
| experiment | 8/27 DT (EcoRI & XbaI) | 3.0 | 8/30 tRNA-Spinach (EcoRI & SpeI) | 12.5 | 3.5
|
| experiment | 8/20 DT (EcoRI & XbaI) | 6.6 | 8/30 RBS-lysis2 (EcoRI & SpeI) | 12.5 | 3.5
|
| experiment | 8/22 J23100 (SpeI & PstI) | -- | RBS-tetR-DT (XbaI & PstI) | -- | 3.5
|
| experiment | 8/22 Plac (SpeI & PstI) | 8.0 | 8/18 RBS-lacZα-DT (XbaI & PstI) | 10 | 3.5
|
Samples were evaporeted used evaporator into about 7 µL
Miniprep
Kojima and Nakamoto
| DNA | concentration[µg/mL] | 260/280 | 260/230
|
| 8/30 Plux-RBS-GFP-DT-(1) | 124 | 1.77 | 1.87
|
| 8/30 Plux-RBS-GFP-DT-(2) | 213 | 1.75 | 1.52
|
| 8/30 Pbad/araC-RBS-RFP | 427 | 1.70 | 1.56
|
| 8/30 Pbad/araC-RBS-RFP | 670 | 1.30 | 0.83
|
| 8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(1) | 332 | 1.73 | 2.04
|
| 8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(2) | 478 | 1.74 | 2.09
|
| 8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(3) | 146 | 1.77 | 2.11
|
| 8/30 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT-(4) | 454 | 1.75 | 2.19
|
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