Team:Glendale CC AZ/Protocols/SurivalGrowth

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Glendale Community College ArizonaGCC

Protocols

iGEM Growth Curve Assay

NaCl Growth Curve Assay

Survival Growth Assay

Alkaline Lysis Plasmid Miniprep

Restriction Digest

DNA Isolation

Bioinformatics

Ligation

Transformation

Survival Growth Assay

Materials 

   -P200 micropipette
   -10µl bacteria
   -90µl LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37°C.


Procedure

1. Label all plates (Keep upside down to avoid condensation on agar)

2. Pipette LB media into tube containing bacteria; aspirate.

3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.

4. Briefly flame the spreader and let cool momentarily.

5. Spread bacteria-LB solution all over plate.

6. Incubate at 37°C for up to 24 hours.

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