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Template:Kyoto/Notebook/Sep 4
From 2013.igem.org
Contents |
Sep 4
Gel Extraction
1
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Pcon | Spe1&Pst1 |
| 3 | Pcon | Spe1&Pst1 |
| 4 | -- | -- |
| 5 | Plac | Spe1&Pst1 |
| 6 | Plac | Spe1&Pst1 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
</div>
2
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Ptet | Spe1&Pst1 |
| 3 | Ptet | Spe1&Pst1 |
| 4 | -- | -- |
| 5 | RBS-tetR-DT | Xba1&Pst1 |
| 6 | RBS-tetR-DT | Xba1&Pst1 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
</div>
Master Plate
| Number | Use LB plate(+Amp) |
|---|---|
| 4 | 8/9 BBa-J23100-3 |
| 5 | 8/9 BBa-J23100-4 |
| 6 | 8/9 BBa-J23100-5 |
Master Plate
| Number | Use LB plate(+Amp) |
|---|---|
| 1 | JM109-1 |
| 2 | JM109-2 |
| 3 | JM109-3 |
| 4 | JM109-4 |
Liquid Culture
| Sample | medium |
|---|---|
| 8/9 BBa-J23100-3 | Plusgrow medium(+Amp) |
