From 2013.igem.org
Sep 4
No name
1
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | Pcon | Spe1&Pst1
|
| 3 | Pcon | Spe1&Pst1
|
| 4 | -- | --
|
| 5 | Plac | Spe1&Pst1
|
| 6 | Plac | Spe1&Pst1
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| （´ω｀） | | | |
|
| （ΦωΦ^） | | | |
|
</div>
No name
2
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | Ptet | Spe1&Pst1
|
| 3 | Ptet | Spe1&Pst1
|
| 4 | -- | --
|
| 5 | RBS-tetR-DT | Xba1&Pst1
|
| 6 | RBS-tetR-DT | Xba1&Pst1
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| （´ω｀） | | | |
|
| （ΦωΦ^） | | | |
|
Master Plate
No name
| Number | Use LB plate(+Amp)
|
| 4 | 8/9 BBa-J23100-3
|
| 5 | 8/9 BBa-J23100-4
|
| 6 | 8/9 BBa-J23100-5
|
Master Plate
Hirano
| Number | Use LB plate
|
| 1 | JM109-1
|
| 2 | JM109-2
|
| 3 | JM109-3
|
| 4 | JM109-4
|
Liquid Culture
Hirano
| Sample | medium
|
| 8/9 BBa-J23100-3 | Plusgrow medium(+Amp)
|
Liquid Culture
Hirano
| Sample | medium
|
| JM109-1(Master plate) | LB medium
|
Colony PCR
Kojima
| Sample | base pair
|
| 8/18 J23100 control -(1) | 1142
|
| 8/18 J23100-RBS-GFP-DT -(1) | 1156
|
| 8/18 J23100-RBS-GFP-DT -(2) | 1156
|
| 8/18 J23100-RBS-luxR-DT -(1) | 1271
|
| 8/18 J23100-RBS-luxR-DT -(2) | 1271
|
| 8/18 J23100-RBS-lacZα-DT -(1) | 670
|
| 8/18 J23100-RBS-lacZα-DT -(2) | 670
|
| 8/18 RBS-lysis3 -(1) | 997
|
| 8/18 RBS-lysis3 -(2) | 997
|
| 8/18 P0440(RBS-tetR-DT) -(1) | 1154
|
| 8/18 P0440(RBS-tetR-DT) -(2) | 1154
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 1min | 30cycles
|
File:Igku Aug19electrophoresis1.png
| Sample | base pair
|
| 8/9 BBa-J23100 3 | --
|
| 8/9 BBa-J23100 4 | --
|
| 8/9 BBa-J23100 5 | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 30s | 30cycles
|
File:Igku Aug19electrophoresis2.png
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