Template:Kyoto/Notebook/Sep 12

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Contents

1 Sep 12

Sep 12

Miniprep

Nakamoto

DNA	concentration[µg/mL]	260/280	260/230
Pcon-pT181atte-DT	293.6	1.88	1.84
Pcon-apt12-1R-DT	303.8	1.75	1.64
Fusion1atte(pSB1c3)	172.0	1.96	2.31		RBS-lisis1-DT	235.1	1.59	1.60		RBS-lisis2-DT	387.7	1.91	2.01		RBS-lisis3-DT	387.9	1.91	2.09		Plux	166.7	1.98	1.63

Electrophoresis

Nakamoto

Lane	Sample
1	100bp ladder
2	9/11 P-RBS-lisis1-DT(Colony PCR prodution)

File:Igku xxxxxx.xxx

Liquid Culture

Nakamoto

Sample	medium
9/10 Pcon-apt12-1R-DT1	Plusgrow medium(+Amp)
9/10 DT-Pcon-pT181atte1	Plusgrow medium(+CP)
9/10 Fusion1-atte(pSB1c3)	Plusgrow medium(+CP)

v>

Electrophoresis

Tatsui

1

} File:Igku xxxxxx.xxx

Electrophoresis

Tatsui

2

Lane	Sample
1	1kb ladder
2	-	3	pSB4K5 NC(EcoR1&Spe1)
4	-	5	-	6	Pcon-RBS-lacz-DT( EcoR1&Spe1)
7	-	8	1kb ladder

} File:Igku xxxxxx.xxx

Lane	Sample
1	1kb ladder
2	-	3	Ptet-pT181anti NC(Spe1&Pst1)
4	-	5	-	6	--
7		8	1kb ladder

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