Template:Kyoto/Notebook/Aug 17

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Aug 17

Nakamoto

Sample	base pair
8/15 BBa_K112806(lysis2) -(1)	828
negative control	--

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	1min	30cycles

Sample	base pair
8/10 BBa_Q0400 -(1)	3995
negative control	--

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	30s	30cycles

Nakamoto

Sample	medium
8/15 BBa_K112806(lysis2) -(1)	Plusgrow medium(+CP)
8/15 BBa_Q04400(tetR) -(1)	Plusgrow medium(+Amp)

Murata,Murakami,Nakamoto,Ashida,Matsumoto and Yoshida

	8/11 J23100 -(2)	SPeI	PstI	Buffer	MilliQ	total
2 cuts	4.0µL	1.0µL	1.0µL	3.0µL	21µL	30µL
1 cut	1.3µL	0.2µL	--	1.0µL	7.5µL	10µL
1 cut	1.3µL	--	0.2µL	1.0µL	7.5µL	10µL
NC	1.3µL	--	--	1.0µL	7.7µL	10µL

	8/15 B0034(RBS) -(2)	SPeI	PstI	Buffer	MilliQ	total
2 cuts	6.0µL	1.0µL	1.0µL	3.0µL	19µL	30µL
1 cut	2.0µL	0.2µL	--	1.0µL	6.8µL	10µL
1 cut	2.0µL	--	0.2µL	1.0µL	6.8µL	10µL
NC	2.0µL	--	--	1.0µL	7.0µL	10µL

	8/17 K117000(lysis1) -(1)	XbaI	PstI	BSA	Buffer	MilliQ	total
2 cuts	2.0µL	1.0µL	1.0µL	3.0µL	3.0µL	20µL	30µL
1 cut	1.0µL	0.2µL	--	1.0µL	1.0µL	6.8µL	10µL
1 cut	1.0µL	--	0.2µL	1.0µL	1.0µL	6.8µL	10µL
NC	1.0µL	--	--	1.0µL	1.0µL	7.0µL	10µL

	8/17 K117000(lysis1) -(1)	XbaI	PstI	BSA	Buffer	MilliQ	total
2 cuts	2.0µL	1.0µL	1.0µL	3.0µL	3.0µL	20µL	30µL
1 cut	1.0µL	0.2µL	--	1.0µL	1.0µL	6.8µL	10µL
1 cut	1.0µL	--	0.2µL	1.0µL	1.0µL	6.8µL	10µL
NC	1.0µL	--	--	1.0µL	1.0µL	7.0µL	10µL

Nakamoto

Number	Use LB plate (+CP)
1	BBa_K112806(lysis2) -(1)

Number	Use LB plate (+Kana)
1	BBa_Q04400(tetR) -(1)