Team:Wisconsin-Madison/parts 3

Text Plasmids

In addition to the Gibson enzyme plasmids, we have also provided test plasmids that can be used to test a gibson reaction. The product test plasmid is a UV green fluorescent protein from a p70RG plasmid inserted in a pMSBmCherry (RFP) plasmid backbone. The two parts can be amplified by PCR from their respective plasmids and then used in a gibson assembly reaction. Colonies with the PCR products will be UV Green fluorescent when induced with IPTG if the assembly is correct and false positives will have much lower UV fluorescence, the p70RG false positives having no color and the pMSBmCherry false positives being noticeably red.