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From 2013.igem.org

{{Team:Purdue/Content|title=Bicistronic Design Tag|content=

Overview

Despite having progressed extensively in the field of synthetic biology in terms of DNA synthesis, analysis and transplanting, we still cannot reliably, quantitatively measure expression of new genetic constructs. We engineered an expression cassette to control transcription and translation initiation which can be reused in new genetic contexts. The Bicistronic design(BCD) consists of two Shine-Dalgarno sequences in its translation element which when combined with indiscriminate gene of interests are known to reliably express within twofold of the relative target expression window. }}