mRFP Quantification

To Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.

File:PArsrfp.jpg

As it is shown in the figure below, with 1000 ppb of arsenic a typical transcriptional induction is observed over time.