From 2013.igem.org
Sep 17
Liquid Culture
Tatsui and Hirano
Sample | medium
|
Spi-DT | LB medium(+CP)
|
Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp)
|
PCR
Hirano
Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total
|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5
|
PreDenature | Denature | Annealing | Extension | cycle
|
96°C | 96°C | 50°C | 60°C | --
|
2m | 10s | 5s | 4m | 30cycles
|
Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total
|
1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5
|
PreDenature | Denature | Annealing | Extension | cycle
|
96°C | 96°C | 50°C | 60°C | --
|
2m | 10s | 5s | 4m | 30cycles
|
Ligation
No Name
state | Vector | Inserter | Ligation High ver.2
|
8/28 | name | xx µg/mL | name | xx µg/mL | xx µg/mL
|
NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL
|