Template:Kyoto/Notebook/Sep 17

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Contents

Sep 17

Liquid Culture

Tatsui and Hirano

Samplemedium
Spi-DTLB medium(+CP)
Pcon-Spi-DT,Pcon-pT181-anti-spi-DTLB medium(+Amp) 

PCR

Hirano

Pcon-tet ap-DT(VF)BigDye5x bufferprimer(VF)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles
Pcon-tet ap-DT(VR)BigDye5x bufferprimer(VR)MilliQtotal
1.30.51.750.50.4510.5
PreDenatureDenatureAnnealingExtensioncycle
96°C96°C50°C60°C--
2m10s5s4m30cycles

Ligation

No Name

stateVectorInserterLigation High ver.2
8/28 namexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

Gel Extraction

No Name

LaneDNAEnzyme
11kbpladder--
2Pt181 atte -(2)E+S
3
4

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)