From 2013.igem.org
Sep 17
Liquid Culture
Tatsui and Hirano
| Sample | medium
|
| Spi-DT | LB medium(+CP)
|
| Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp)
|
PCR
Hirano
| Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total
|
| 1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 96°C | 96°C | 50°C | 60°C | --
|
| 2m | 10s | 5s | 4m | 30cycles
|
| Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total
|
| 1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 96°C | 96°C | 50°C | 60°C | --
|
| 2m | 10s | 5s | 4m | 30cycles
|
Ligation
No Name
| state | Vector | Inserter | Ligation High ver.2
|
| 8/28 | name | xx µg/mL | name | xx µg/mL | xx µg/mL
|
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL
|
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