Template:Kyoto/Notebook/Sep 11

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Sep 11

Tatsui

pKaiBC(200p)	10*buffer KOD plus	dNTPs	MgSO4	primer mix(F&R)	KOD plus	MilliQ	total
	2.5	2.5	1.5	1.5	0.5	16		25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	60°C	68°C	--
2min	10sec	30sec	30sec

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