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• iGEM 2013

Phage Purification March - April Notebook: Experiments

Overview March-April May-June July-August September-October

4.5 Phage Spot Test I) Purpose Test for viable phage. II) Expected Outcome Plaques in the bacteria where phage can infect and kill. III) Reagants Used BL21 1x LB top agar Phage: 14 T3  : 13 T5  : 10 T2  : 20OX171  : 40ØX174 IV) Actual Procedure We plated together .5mL of BL21 with 1X LB TA. We separated the plates into different segments and then spot tested the phage. The plates were then put in a 37°C incubator V) Results No plaques were seen on a uniform bacterial lawn. The control was clear of any contamination or plaques. The phage were not viable.