Template:Kyoto/Notebook/Sep 16

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Contents

1 Sep 16

Sep 16

Liquid Culture

No name

Sample	medium
pSB1C3+P-KaiBC①	Plusgrow medium(+CP)
pSB1C3+P-KaiBC②	Plusgrow medium(+CP)

Colony PCR

No name

Sample	base pair
pSB1C3+P-KaiBC①	283
pSB1C3+P-KaiBC②	283

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
2min	30s	30s	18s	30cycles

Gel Extraction

じっけんしゃ

Lane	DNA	Enzyme
1	1kb ladder
2	pT181 attenuator	X+P
3	pT181 attenuator	X+P
4	pT181 attenuator	X+P
6	pSB1C3	E+S
7	pSB1C3	E+S
8	pSB1C3	E+S
10	pSB4K5	X+P
11	pSB4K5	X+P
12	pSB4K5	X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	concentration[µg/mL]	260/280	260/230
pT181 attenuator(X+P)	8.2	1.62	0.11
pSB1C3(E+S)	49.5	1.48	0.18
pSB4K5(X+P)	18.1	1.73	0.57
DT(S+P)	70.4	1.36	1.02
aptamer12-1R-DT(S+P)	42.0	1.82	0.47
spinach-DT(S+P)	36.8	1.79	0.61

Miniprep

No name

DNA	concentration[µg/mL]	260/280	260/230
Dx-lux1	136.7	1.46	1.28
RBS-laczα-DT	237.6	1.88	1.70
pSB4K5	148.4	1.55	1.09
Pcon-KBS-tetR-DT	126.6	1.73	1.36
9/11Plac1	160.0	1.78	1.28
9/15Plac2	144.0	1.73	1.81
9/14Plac332.5	1.51	2.33
Ptet-RBS-laczα-DT	231.3	1.84	1.57

PCR

No name

9/6pT181 anti psB1C3(1)	KOD plus	10x buffer	dNTP	MgSO4	GG1_1C3_fwd primer	GG2_1C3_rev primer	MilliQ	total
1	0.5	2.5	2.5	1.5	0.75	0.75	15.5	25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	57°C	68°C	30cycles

9/6 12-1R aptamer-DT	KOD plus	10x buffer	dNTP	MgSO4	GG1_1C3_fwd primer	GG1_1C3_rev primer	MilliQ	total
1	0.5	2.5	2.5	1.5	0.75	0.75	15.5	25

PreDenature	Denature	Annealing	Extension	cycle
94°C	98°C	57°C	68°C	30cycles

Electrophoresis

じっけんしゃ

Lane	Sample	Enzyme1	Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

じっけんしゃ

	DNA	restricion enzyme	restriction enzyme	Buffer	BSA	MilliQ	total
2 cuts	（°°） µL	µL	µL	µL	µL	µL	µL
NC	µL	µL	µL	µL	µL	µL	µL

Ligation

じっけんしゃ

state	Vector		Inserter		Ligation High ver.2
experiment	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

PCR

No name

genome DNA	KOD plus	10x buffer	dNTP	MgSO4	SasA_fwd primer	SasA_rev primer	MilliQ	total
	0.5	2.5	2.5	1.5	0.75	0.75		25

PreDenature	Denature	Annealing	Extension	cycle
°C	°C	°C	°C	--
（◎Д◎）

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