Template:Kyoto/Notebook/Aug 10

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Contents

1 Aug 10

Aug 10

Liquid Culture

Nakamoto

Sample	medium
8/9 BBa-J23106	LB(+amp)
8/9 BBa-J23100-1	LB(+amp)
8/9 BBa-J23100-2	LB(+amp)
8/9 BBa-J44000-1	LB(+amp)
8/9 BBa-J44000-2	LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate

Nakamoto & Tatsui

volume	200ml
Bacto T2ypton	2g
Bacto yeast extract	1g
NaCl	1g
0.4µ NaOHaq	50µL
Agar Pouder	2g

Transformation

Gel Making

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