Template:Kyoto/Notebook/Sep 4

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Contents

1 Sep 4

Sep 4

Gel Extraction

No name

1

Lane	DNA	Enzyme
1	100bp ladder	--
2	Pcon	SpeI&PstI
3	Pcon	SpeI&PstI
4	--	--
5	Plac	SpeI&PstI
6	Plac	SpeI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
Pcon	--	13.2	1.99	-1.64
Plac	--	15.3	2.07	-o.39

</div>

No name

2

Lane	DNA	Enzyme
1	100bp ladder	--
2	Ptet	SpeI&PstI
3	Ptet	SpeI&PstI
4	--	--
5	RBS-tetR-DT	XbaI&PstI
6	RBS-tetR-DT	XbaI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
Ptet	--	30.1	1.97	-0.97
RBS-tetR-DT	--	3.0	2.44	0.88

Gel Extraction

No name

Lane	DNA	Enzyme
1	100bp ladder	--
2	spinach-DT	XbaII&PstI
3	spinach-DT NC	XbaII&PstI
4	RBS-lacZα-DT	XbaI&PstI
5	RBS-lacZα-DT NC	XbaI&PstI	6	100bp ladder	--

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
Pcon	--	13.2	1.99	-1.64
Plac	--	15.3	2.07	-o.39

</div>

No name

Master Plate

No name

Number	Use LB plate (+Amp)
4	8/9 BBa_J23100-3
5	8/9 BBa_J23100-4
6	8/9 BBa_J23100-5

Master Plate

Hirano

Number	Use LB plate
1	JM109-1
2	JM109-2
3	JM109-3
4	JM109-4

Liquid Culture

Hirano

Sample	medium
8/9 BBa_J23100-3	Plusgrow medium(+Amp)

Liquid Culture

Hirano

Sample	medium
JM109-1(Master plate)	LB medium

Colony PCR

Hirano

Sample	base pair
8/9 BBa_J23100 3	--
8/9 BBa_J23100 4	--
8/9 BBa_J23100 5	--

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	30s	30cycles

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