Template:Kyoto/Notebook/Sep 7

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Sep 7

No Name

Name	concentration[µg/mL]	260/280	260/230
apt 12_1R-DT	12.7	1.62	5.74
RBS-lysis2-DT	6.7	1.71	5.46
Pλ-luvI	8.1	1.76	2.25

No name

DNA	concentration[µg/mL]	260/280	260/230
RBS-lysis1-DT	195.3
RBS-lysis3-DT	255.5	1.91	2.04
Ptet-pT18	183.6	1.85	1.48
Pcon-RBS-tetR-DT	134.3	1.94	2.20
Ptet-RBS-lacZα-DT	67.7	1.92	1.79
Pcon-pT181 attenuator	232.3	1.95	1.90
pSB4K5	237.0	1.61	1.50
pT181 antisense(pSB1C3)	256.4	1.63	1.78
apt12_1R(pSB1C3)	230.0	1.98	2.28

No Name

state	Vector		Inserter		Ligation High ver.2
experiment	9/6 Plux (SpeI&PstI)	2 µL	9/7 RBS-lysis2-DT (EcoRI&XbaI)	µL	3.5 µL
experiment	9/6 Plac (SpeI&PstI)	µL	9/7 RBS-lysis2-DT (EcoRI&XbaI)	µL	3.5 µL
experiment	9/6 Pcon-RBS-luxR-DT (EcoRI&XbaI)	1.9 µL	9/6 Plux-RBS-GFP-DT(SpeI&PstI)	2.2 µL	3.5 µL
experiment	9/6 Pbed/araC (SpeI&PstI)	1.6 µL	9/5 RBS-luxI-DT (EcoRI&XbaI)	16 µL	3.5 µL
experiment	9/6 Plac (SpeI&PstI)	1.5 µL	9/5 pT181 antisense (EcoRI&XbaI)	2.0 µL	3.5 µL
experiment	9/6 Pcon (SpeI&PstI)	3.0 µL	9/5 RBS-luxI-DT (EcoRI&XbaI)	10 µL	3.5 µL

No name

Sample	base pair
9/5 Plac-spinarch-DT-13~20
9/5 Plac-pT181 attenuator-13~20

PreDenature	Denature	Annealing	Extension	cycle
94　°C	94　°C	55　°C	60　°C	--
5min	30s	30s	42s	30cycle