Template:Kyoto/Notebook/Sep 15

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Contents

Sep 15

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
Pλ-luxI225　1.59　0.83

Liquid Culture

No name

Samplemedium
9/13 Pλ-luxI-1
9/7 pSB4K5-1
9/11 Plac-3

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experiment9/13 Pcon(SpeI & PstI)3.6 µL9/12 RBS-lacZα-DT(XbaI & PstI)4.7 µL8 µL

Restriction Enzyme Digestion

じっけんしゃ

DNAEcorISpeIBufferBSAMilliQtotal
9/7Pcon-RBS-tetR-DT (EcoRI & SpeI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


DNAEcoRISpeIBufferBSAMilliQtotal
9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


DNAEcoRISpeIBufferBSAMilliQtotal
9/12 Pcon-apt12_1R-DT(EcoRI & SpeI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


DNAEcoRISpeIBufferBSAMilliQtotal
9/8 Pcon-spin-DT (EcoRI & SpeI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


DNAXbaIPstIBufferBSAMilliQtotal
9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


DNAXbaIPstIBufferBSAMilliQtotal
8/17 RBS-lacZα-DT (XbaI & PstI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


DNAEcoRIXbaIBufferBSAMilliQtotal
9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL


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