Template:Kyoto/Notebook/Sep 15

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Sep 15

No name

DNA	concentration[µg/mL]	260/280	260/230
Pλ-luxI	225	1.59	0.83

No name

じっけんしゃ

state	Vector		Inserter		Ligation High ver.2
experiment	9/13 Pcon(SpeI & PstI)	3.6 µL	9/12 RBS-lacZα-DT(XbaI & PstI)	4.7 µL	8 µL

No Name

	DNA	EcorI	SpeI	Buffer	BSA	MilliQ	total
9/7Pcon-RBS-tetR-DT (EcoRI & SpeI)	15 µL	1 µL	1 µL	3 µL	3 µL	6 µL	30 µL
NC	0.7 µL	0 µL	0 µL	1 µL	1 µL	7.3 µL	10 µL

	DNA	EcoRI	SpeI	Buffer	BSA	MilliQ	total
9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI)	6.8 µL	1 µL	1 µL	3 µL	3 µL	15.2 µL	30 µL
NC	0.3 µL	0 µL	0 µL	1 µL	1 µL	7.7 µL	10 µL

	DNA	EcoRI	SpeI	Buffer	BSA	MilliQ	total
9/12 Pcon-apt12_1R-DT(EcoRI & SpeI)	6.6 µL	1 µL	1 µL	3 µL	3 µL	15.4 µL	30 µL
NC	0.3 µL	0 µL	0 µL	1 µL	1 µL	7.7 µL	10 µL

	DNA	EcoRI	SpeI	Buffer	BSA	MilliQ	total
9/8 Pcon-spin-DT (EcoRI & SpeI)	7.6 µL	1 µL	1 µL	3 µL	3 µL	15.4 µL	30 µL
NC	0.3 µL	0 µL	0 µL	1 µL	1 µL	7.7 µL	10 µL

	DNA	XbaI	PstI	Buffer	BSA	MilliQ	total
9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI)	7.2 µL	1 µL	1 µL	3 µL	3 µL	14.8 µL	30 µL
NC	0.4 µL	0 µL	0 µL	1 µL	1 µL	7.6 µL	10 µL

	DNA	XbaI	PstI	Buffer	BSA	MilliQ	total
8/17 RBS-lacZα-DT (XbaI & PstI)	5.3 µL	1 µL	1 µL	3 µL	3 µL	16.7 µL	30 µL
NC	0.5 µL	0 µL	0 µL	1 µL	1 µL	7.5 µL	10 µL

	DNA	EcoRI	XbaI	Buffer	BSA	MilliQ	total
9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI)	2.9 µL	1 µL	1 µL	3 µL	3 µL	18.5 µL	30 µL
NC	1.5 µL	0 µL	0 µL	1 µL	1 µL	7 µL	10 µL

No Name

Lane	Sample	Enzyme1	Enzyme2
1,14
2	Pcon-RBS-tetR-DT	EcoRI	SpeI
3	Pcon-RBS-tetR-DT
4	Pcon-pT181 attenuator-DT	EcoRI	SpeI
5	Pcon-pT181 attenuator-DT
6	Pcon-apt12-1R-DT	EcoRI	SpeI
7	Pcon-apt12-1R-DT
8	Pcon-spin-DT	EcoRI	SpeI
9	Pcon-spin-DT
10	Pcon-pT181 antisense-spin-DT	XbaI	PstI
11	Pcon-pT181 antisense-spin-DT
12	RBS-lacZα-DT	XbaI	PstI
13	RBS-lacZα-DT
15	Ptet-RBS-lacZα-DT	EcoRI	XbaI
16	Ptet-RBS-lacZα-DT
17	1kbp ladder

No Name 1

2

Name	concentration[µg/mL]	260/280	260/230
9/15 Ptet-RBS-lacZα-DT (EcoRI & SpeI)	1.9	1.08	0.77
9/15 Pcon-RBS-tetR-DT (EcoRI & SpeI)	5.9	1.30	0.17
9/15 RBS-lacZα-DT (XbaI & PstI)	1.0	0.85	0.44
Pcon-pT181 anticodone-spin-DT (XbaI & PstI)	1.3	0.95	0.28
Pcon-spin-DT	0.9	1.37	0.05
Pcon-apt12_1R-DT	1.7	1.42	0.29
Pcon-pT181 attenuator-DT (EcoRI & SpeI)	2.1	1.53	0.46

No Name

state	Vector		Inserter		Ligation High ver.2
experiment	9/13 pSB1C3 EcoRI & SpeI	4.7 µL	9/15 Pcon-RBS-tetR-DT EcoRI & SpeI	1.9 µL	xx µL
experiment	9/13 pSB1C3 EcoRI & SpeI	4.7 µL	9/15 Pcon-spin-DT EcoRI & SpeI	4.7 µL	xx µL
experiment	9/13 pSB1C3 EcoRI & SpeI	4.7 µL	9/15 Pcon-apt12_1R-DT EcoRI & SpeI	2.0 µL	xx µL
experiment	9/13 pSB1C3 EcoRI & SpeI	4.7 µL	9/15 Pcon-pT181 attenuator-DT EcoRI & SpeI	3.0 µL	xx µL
experiment	9/13 pSB1C3 XbaI & PstI	1.9 µL	9/15 Pcon-pT181 anticodone-spin-DT XbaI & PstI	4.4 µL	xx µL
experiment	9/13 pSB1C3 EcoRI & SpeI	4.7 µL	9/3 pT181 attenuator EcoRI & SpeI	xx µL	3.8 µL
experiment	9/6 Pcon SpeI & PstI	1.4 µL	9/15 RBS-lacZα-DT XbaI & PstI	xx µL	2.0 µL
experiment	9/14 pSB 4k5 EcoRI & SpeI	µL	9/15 Pcon-spin-DT EcoRI & SpeI	xx µL	9.4 µL
experiment	9/15 Peet-RBS-lacZα-DT EcoRI & XbaI	2.6 µL	Pcon-RBS-tetR-DT EcoRI & SpeI	1.5 µL	xx µL