Team:Carnegie Mellon/KillerRed

Figure 1: Crystal Structure of KillerRed (PDB ID: 3GB3) with the QYG chromophore shown^2,3,4

Figure 2: ß-barrel shape of a KillerRed monomer.^2,3,4 PDB ID: 2WIQ

KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from anm2CP to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates in cells. Expression of KillerRed and irradiation with light may act a kill-switch for biosafety applications.

The structure of KillerRed has been analyzed and the structural basis for its toxicity has been explored. The relevant PDB IDs are 2WIQ and 2WIS for the fluorescent and dark states, respectively.

The surface of KillerRed is predominantly negatively charged. As shown in figures 3-6

Figure 3: Electrostatic surface at 1.4 Å of KillerRed dimer (from -10 kcal/(mol*e) in red to +10 kcal(mol*e) in blue).^3,4 PDB ID: 2WIQ

Figure 4: Electrostatic surface at 1.4 Å of KillerRed dimer (from -10 kcal/(mol*e) in red to +10 kcal(mol*e) in blue).^3,4 PDB ID: 2WIQ

Figure 5: Electrostatic surface at 1.4 Å of KillerRed dimer (from -10 kcal/(mol*e) in red to +10 kcal(mol*e) in blue).^3,4 PDB ID: 2WIQ

Figure 6: Electrostatic surface at 1.4 Å of KillerRed dimer (from -10 kcal/(mol*e) in red to +10 kcal(mol*e) in blue).^3,4 PDB ID: 2WIQ

Figure 7: The interface between the homodimers consist primarily of hydrophobic interactions and two hydrogen-bonding histidine residues. The histidines and their hydrogen bonds are shown. PDB ID: 2WIQ

Figure 8: The protein produces superoxide anion radicals, which can react with proteins and lipids to deactivate them. KillerRed contains many sulfurous residues (methionines and cysteines) that can act as antioxidants. The large volume of the sulfur atom stabilizes the radical and prevents significant damage to the protein's backbone and side chains.^2,3,4 PDB ID: 2WIQ

Figure 9: This is the water channel that produces the superoxide anion radical when exposed to light. The ribbon structure is shown for reference. Na+ is shown in purple.^2,3,4 PDB ID: 2WIQ

Figure 10: This is the water channel that produces the superoxide anion radical when exposed to light. The hydrogen bonds are shown as pseudobonds and surrounding residues are shown and labeled.^2,3,4PDB ID: 2WIQ

References

¹ Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175

² Sergei Pletnev, Nadya G. Gurskaya, Nadya V. Pletneva, Konstantin A. Lukyanov, Dmitri M. Chudakov, Vladimir I. Martynov, Vladimir O. Popov, Mikhail V. Kovalchuk, Alexander Wlodawer, Zbigniew Dauter, and Vladimir Pletnev.
Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed
Journal of Biological Chemistry 2009 284: 32028-32039. First Published on September 8, 2009, doi:10.1074/jbc.M109.054973

³Molecular graphics and analyses were performed with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, funded by grants from the National Institutes of Health National Center for Research Resources (2P41RR001081) and National Institute of General Medical Sciences (9P41GM103311).

⁴Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE.
UCSF Chimera--a visualization system for exploratory research and analysis.
Journal of Computational Chemistry. 2004 Oct;25(13):1605-12.

⁵Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Yoshiyuki A, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Sci. Rep. 2013/09/17/online. 3. Macmillan Publishers Limited. All rights reserved. http://dx.doi.org/10.1038/srep02629