Template:Kyoto/Notebook/Sep 3

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Contents

1 Sep 3

Sep 3

Electrophoresis

Kozima

Lane	Sample
1	100bp ladder
2	pT181 attenuater(2)-DT2(738)
3	Ptet-RBS-lacZα-DT-2(756)
4	Ptet-RBS-lacZα-DT-3
5	Ptet-RBS-lacZα-DT-4
6	Ptet-RBS-lacZα-DT-5
7	Ptet-RBS-lacZα-DT-6
8	RBS-lysis2-DT-2(985)
9	RBS-lysis2-DT-3
10	100bp ladder

File:Igku xxxxxx.xxx

Miniprep

Tatsui

DNA	concentration[µg/mL]	260/280	260/230
9/2 14:00 pT181 attenuator	418.4	1.82	1.80
9/2 14:00 pT181 antisense	362.2	1.92	1.96
9/2 14:00 Spinach	438.3	1.86	1.99
9/2 14:00 tetR-apt 12_1R	567.5	1.90	1.99
9/2 21:00 pT181 attenuater	320.9	1.90	2.15
9/2 21:00 Spinach	457.0	1.88	2.24

Liquid Culture

Kozima

Sample	medium
9/2 Spinach-DT	plusgrow(CP)4ml

Restriction Enzyme Digestion

PCR

Mutatation PCR

Colony PCR

Restriction Enzyme Digestion

Gel Extraction

Liquid Culture

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