Team:Paris Bettencourt/Notebook/TB-ception/Friday 13th September.html

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TB-ception

Friday 13th September


A lot of macrophages died (in suspension) because we forgot to add glutamine and pyruvate sodium. We repeated the experiment with the complete media (but again without antibiotics).


• Results for the GA: because the Chl plates were off, I have to repeat everything for biobricking TDMH and for the pACYC derived backbone. The cloning for pCDF and pColA worked - patched the colonies, start liquid cultures.

• Launch NEB Turbo to make competent cells.

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