Fluorescence measurements Measuring of mRFP with Tecan Infinite® M200 platereader Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings: 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) Measurement mode: Top Excitation: 584 nm Emission: 620 nm Number of reads: 25 Manual gain: 100 Integration time: 20 µs

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