Team:NTNU-Trondheim/Protocols

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Contents

1 Laboratory Protocols
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Laboratory Protocols

Transformation

Official iGEM [http://parts.igem.org/Help:Protocols/Transformation Transformation protocol].

We used this protocol with these modifications:

Heat-shock 45 seconds instead of 60 seconds
Incubation for min 1 hour in shaker at 37°C, not 2 hours.
LB medium instead of SOC.

Transformed cells

Use a sterile toothpick, scratch a single colony from the transformed cells. Place the toothpick into a plastic tube with 4 mL sterile liquid medium with the appropriate antibiotic(s). Cap the tube and incuate at 37°C with shaking.

DNA Isolation

We use the Promega Wizard Plus SV Minipreps DNA Purification System A1460 [http://www.promega.co.uk/~/media/Files/Resources/Protocols/Technical%20Bulletins/0/Wizard%20Plus%20SV%20Minipreps%20DNA%20Purification%20System%20Protocol.pdf Miniprep protocol].

more protocols

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