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Team:SYSU-China/Project/Design
From 2013.igem.org
ipsc
suicide gene?
Why suicide gene?
In our project, we try to build a circuit that can prevent the iPSC-differentiated tissues from tumor formation. Instead of using some medicines to kill them, like the traditional gene therapies did, what we want to achieve here is to make the tumor cells kill themselves, in other word, automatically commit suicide. So we decided to use an exogenous suicide gene.
The ideal suicide genes that fit our design here will be genes that can successfully induce apoptosis in cells and do not introduce any harmful effect to normal tissues. However, many apoptosis pathways are reported to be blocked in tumor cells. From recent reports and papers, we found out that there have been many genes reported to have important roles in mammalian cell apoptosis. After a long time comparison and consideration, our final candidates of suicide gene are listed below:
- hBax & hBax mutant
- delta TK
- caspase3
- rip1
- rip3
- apoptin
The introduction of each genes about its suicide mechanisms and the reasons why we choose them are as follows:
References
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Sun Yat-Sen University, Guangzhou, China
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