Team:Shenzhen BGIC ATCG/results

The Magic

Our project “Cell Magic", a complex work has been completed partly,due to the time limitation. The results we have gotten are listed below:

We verified the Clb6 promoter, which is supposed to be expressed in G1 phase.

Promoter Verification

Using the GFP as reporter and morphological alteration as cell cycle representation, we verified the Clb6 can be activated in G1 phase in the yeast.

As shown in the picture in normal lights, there are some yeast were budding. These budded yeast cells and small size budding yeasts (within the red circles) are assumed in the G1 phase of cell cycle. And the next picture was taken under activation light, thus the green lights can representing the Clb6 promoter expressing. The two pictures indicated the Clb6 were expressed in the G1 phase as we expected when designing the experiments

PS: we modified the contrast ratio to lower the lights of neighbor cells, thus our results looking better.

预留橙色cln3图

预留流式细胞仪图

Reporter Locating

预留荧光蛋白图

The four pictures shows the reporters can rightly locate to Mitochondria, Nucleus and Vacuole，respectively. Picture A is the negative control, all yeast cells are lighted with GFP. And figure B is the reporter to the mitochondria, we can saw there are several light spots in one cells. Figure C is the reporter located in nucleus, the green spots are small and there is only one in a yeast cell.The last picture shows the reporter of Vacuolar membrane, the green lights were discrete in cells which was as expected.

Degradation Rate

In every curcuits measurement, we firstly test the growth curve through detecting the absorbance.

From right to left, the negative control,BBa_K1051257, BBa_K1051258, BBa_K1051259, Positive Control. As the pictures showed, the lights of RFP within three degradation tags are decreasing.

The test results of BBa_K1051258. A:No exciting lights B. Powerful exciting lights C Weak exciting lights.

In picture, there are only obvious lights in the picture B, indicated the degradation rates are working

预留酶标仪结果图和柱状图，降解效率换算

The test results of BBa_K1051258 in chip. A,LB medium,O minuts; B, IPTG medium,9minutes; C,IPTG medium, 15 minutes Using the IPTG medium, the new RFP expression was stopped and we can regard the lights as degradation tags'effecience. Obviously, the lights are decreasing along with the time. Thus, it indicates that the degradation tags work effectively

预留半衰期换算

Cell Synchronization

Alternative Splicing

intron方案”进化“说明