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Team:Bonn
From 2013.igem.org
Regulating protein activity is important throughout lifescience research. Even though there are several systems available for regulation of proteins all suffer from certain disadvantages.
Aiming to overcome all of these drawbacks we engineer a novel system for light dependend regulation of protein degradation resulting in a rapid change of protein activity.
Our system can be not only of great importance for fundamental science to investigate protein function but also for accurate control of bioreactors and biological machines.
Project
Making versatile control of biological machines easily possible for everybody we engineer a system for
light dependend control of protein activity per protein degradation.
For protein degradation we harness the ClpXP protease system of prokaryotes. This system enables to
activate degradation of a peptid tag (ssrA) fused protein upon induction with the adaptor protein SspB.
Using a Split version of SspB, protein degradation is activated through heterodimerisation of both SspB
parts.
To obtain light inducible degradation we use a light dependend heterodimerisation system. The light
sensitive part of this system is LOV2 (A. Sativa).
Modelling of the light dependency of protein degradation will even make simple and accurate control of
protein activity for any desired level of activity possible.
Find out more about
the ClpXP protease System
our Comparison of several light sensitive systems
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Background
To enable protein degradation we decided on one proteases system (ClpXP), which allows specific degradation of proteins, is highly conserved and well established.
For light dependency we compared several systems to finally find the best suited one. We created a comprehensive overview of all systems making artificially light control possible so that everyone can easily choose the optimal system for his approach.
Find out more about
the ClpXP protease System
our Comparison of several light sensitive systems
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Human Practice
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Team
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