"
Team:Groningen/Labwork/26 September 2013
From 2013.igem.org
Mirjam
Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.
Claudio & Sander
First we now realize that iodine kills Bacillus subtilis.6 colonies from each Bacillus subtilis transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.
Inne
Ran a 0.8% agarose gel to check the results from the colony PCR, 12 samples were tested. mixture 5uL sample + 5uL 2x loading dye. gel ran at 90 Volts for 35 minutes.Gel composition:
Gel 2
| Ladder | 1 | 2 | 3 | 4 | 5 | 6 |
| Ladder | 7 | 8 | 9 | 10 | 11 | 12 |
iGEM 2013 Groningen
|
|
|
|
 |
|
