Experiment trainingWe learnt to culture the 293t cells.We learnt to extract plasmids and transfect it into 293t cells.We learnt to collect exosomes.We learnt to extract RNA from exosomes and cells.We learnt to do RT-PCR and qPCR.
siRNA screening (Failed)14th: We extracted plasmids and cultured the 293t cells.15th: We transfected plasmids into 293t cells.16th: We collected cells and preserved it in Trizol.17th: We extracted RNA, then did RT-PCR with the RNA.18th: We did qPCR with the cDNA we got on 17th April.21th: We reexamined the concentration of RNA, and redid qPCR.
siRNA screening (Failed)27th: We cultured the 293t cells in a 12-well plate.28th: We transfected 293t cells.29th: We collected cells and extracted RNA from it.30th: We did RT-qPCR with the RNA we extracted on 29th April.1st: We did qPCR with the cDNA we got on 30th April.
siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)8th: We extracted plasmids of 3 kinds of siRNA.9th: We cultured 293t cells in eight D10 dishes and a 12-well plate.10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively.11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate.12th: We extracted RNA from cells and exosomes.13th: We did RT-PCR and qPCR.
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