Team:Carnegie Mellon/Week15

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Killer Red

Saturday, September 14th

Condition Viable count
KillerRed Unexposed 7.6 x 108
KillerRed Exposed 1 3.4 x 108
KillerRed Exposed 2 5.3 x 108
KillerRed Exposed 3 2.9 x 108
KillerRed Exposed (Avg) 3.9 x 108 ± 1.3 x 108

Condition Viable count
RFP Unexposed 3.9 x 108
RFP Exposed 1 2.4 x 108
RFP Exposed 2 3.5 x 108
RFP Exposed 3 2.0 x 108
RFP Exposed (Avg) 2.6 x 108 ± .78 x 108

RFP 33% ± 20% killed
KillerRed: 49% ± 17% killed

Thursday , September 19

Filled 96-well plate with 24 samples (two rows) each of KR and RFP plasmid and lysogen. One row of each exposed and unexposed. Exposed by doing 25 kinetic cycles on Tecan, 200 reads per cycle. Results inconclusive… protein may have been maturing during the experiment since cultures were induced the same day.

Friday, September 20

Reread plate which had been stored overnight at 4degC.