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From 2013.igem.org

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  • Overview
  • Cell Polarization
  • Ion Pumps
  • Biofilm Membrane
• Notebook
  • Notebook 1: Polarization
  • Notebook 2: Pumps
  • Notebook 3: Biofilm
  • Safety
• Team
  • Team Members
• Acknowledgements

**Safety forms were approved by David Lloyd and Julie McNamara.

1) Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:

• Species: E. coli
  
• Strain: TG1 and Epi300
  
• Risk Group: 1
  
• Risk Group Source Link: http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli
  
• Disease risk to humans? If so, which disease?: "E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm).
  

• Species: Caulobacter crescentus
  
• Strain: CB15 and CB15N
  
• Risk Group: 1
  
• Risk Group Source Link: http://www.baua.de/en/Topics-from-A-to-Z/Biological-Agents/TRBA/pdf/TRBA-466.pdf?__blob=publicationFile&v=3
  
• Disease risk to humans? If so, which disease?: Ubiquitous presence in environmental water supplies including human drinking water and swimming pools. Single case reported of Caulobacter acting as a pathogen in a human.
  

2) Highest Risk Group Listed: 1 See pg 52 of Caulobacter PDF document above for risk group for Caulobacter crescents.