From 2013.igem.org
Sep 27
Kojima
| Lane | DNA | Enzyme
|
| 1 | 1kbp ladder | --
|
| 2 | pSB1C3 | EcoRI+SpeI
|
| 3
|
| 4
|
| 6 | Pcon-attenuator-aptmer-DT | EcoRI+SpeI
|
| 7
|
| 8
|
| 10 | Pcon-attenuator-aptmer-DT | EcoRI+SpeI
|
| 11
|
| 12
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| Pcon-attenuator-DT(EcoRI+SpeI) | 6.8 | 1.98 | 0.43
|
| pT181-attenuator(EcoRI+SpeI) | 7.5 | 1.84 | 0.06
|
| pSB1C3(EcoRI+SpeI) | 17.7 | 1.97 | 0.71
|
Electrophoresis
Liquid Culture
Ligation
じっけんしゃ
| state | Vector | Inserter | Ligation High ver.2
|
| experiment | name | xx µL | name | xx µL | xx µL
|
| NC | name | xx µL | name | xx µL | xx µL
|
Liquid Culture
No name
| Sample | medium
|
| Pcon-spinach-DT | plusgrow Amp
|
| Pcon-tetRaptamer-DT | plusgrow Amp
|
| Pcon-antisense-spinach-DT | plusgrow Amp
|
| spinach-DT | plusgrow CP
|
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| 9/26 Pcon-GFP-DT | 423.0 | 1.83 | 1.46
|
| 9/26 Pcon-GFP-DT K | 298.7 | 1.82 | 1.50
|
Electrophoresis
じっけんしゃ
| Lane | Sample
|
| 1 | 9/27 Pcon-GFP-DT(RNA)
|
| 2 | 1kb ladder
|
| 3 | 9/27 Pcon-GFP-DT K(RNA)
|
File:Igku xxxxxx.xxx
Miniprep
No name
| DNA
|
| Pcon-aptamer-DT
|
| Pcon-spinach-DT
|
| Pcon-antisense
|
| Pcon-tetR-DT
|
| Pcon-attenuator-DT
|
| Pcon-antisense-spinach-DT
|
Transformation
No name
| Name1 | Name2 | Sample1(µL) | Sample2(µL) | Competent Cells(µL) | Total(µL)
|
| Pcon-attenuator+RBS-GFP-DT(4K5) | Pcon-attenuator-DT(1A2) | 2 | 1 | 30 | 33
|
| Pcon-attenuator+RBS-GFP-DT(4K5) | Pcon-antisense-spinach-DT(1A2) | 2 | 1 | 30 | 33
|
| Pcon-attenuator+RBS-GFP-DT(4K5) | Pcon-tetR aptamer-DT(1A2) | 2 | 1 | 30 | 33
|
| Pcon-RBS-GFP-DT(4K5) | Pcon-attenuator-DT(1A2) | 2 | 1 | 30 | 33
|
| Pcon-RBS-GFP-DT(4K5) | Pcon-antisense-spinach-DT(1A2) | 2 | 1 | 30 | 33
|
| Pcon-RBS-GFP-DT(4K5) | Pcon-tetR aptamer-DT(1A2) | 2 | 1 | 30 | 33
|
| Pcon-attenuator+RBS-GFP-DT(4K5) | -- | 2 | -- | 20 | 22
|
| Pcon-RBS-GFP-DT(4K5) | -- | 2 | -- | 20 | 22
|
| attenuator(1C3) | -- | 2 | -- | 20 | 22
|
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