Team:KIT-Kyoto/Notebook/ATF1/may

May 16^th

We performed PCR to amplify the ATF1 gene.

Primers:

Forward:5’-TACGCCTCGAGATGAATGAAATCGATGAGAAAAATCAGGCCCC-3’

Reverse:5’-ATATTGCTTAGCAGGGCCTAAAAGGAGAGCTTTGTAAATGGAGCAAAGC-3’

Reaction composition is as follows:

We could not get any PCR product.

May 17^th -20^th

Changed PCR conditions with another DNA template. We performed PCR of the ATF1 gene again.

Primers:

Forward :5’-TACGCCTCGAGATGAATGAAATCGATGAGAAAAATCAGGCCCC-3’

Reverse:5’-ATATTGCTTAGCAGGGCCTAAAAGGAGAGCTTTGTAAATGGAGCAAAGC-3’

Reaction composition is as follows:

We could not get any PCR product.

May 30^th -31^th

We transformed a plasmid carrying the ATF1 gene into E.coli.

This plasmid was obtained from iGEM DNA distributions kit 2012 (plate 2, o-7).

We could see approximately 50 colonies and cultured furthermore.